Tris-glycine-MeOH transfer buffer:
2.42g Tris base
11.26g glycine
200mL methanol (20% final)
water to 1L
TBST:
6.1g Tris base
8.7g NaCl
water to 1L
pH to 7.5 with 1.5mL concentrated HCl
add 5mL 10% Tween-20 (0.05% final)
10X TBST:
61g Tris base
87g NaCl
900mL water
pH to 7.5 with concentrated HCl
add 50mL 10% Tween-20
add water to 1L
autoclave and store at RT indefinitely
Ponceau S:
200mg Ponceau S (Sigma P-3504)
3mL 100% TCA (6.6g TCA in 3mL)
water to 100mL