50X TAE Buffer

for 500mL

121g Tris base in 250mL ddH2O

stir to dissolve

add 28.6mL acetic acid

add 50mL 0.5M EDTA pH 8.0

measure in graduated cylindar and add ddH2O to 500mL

1X TAE Buffer

for 500mL

10mL 50X TAE buffer

490mL ddH2O

1% agarose gel in TAE

for 100mL

1g agarose

100mL 1X TAE

microwave 1 minute to get into solution, do not allow to boil over

if crystals not dissolved, swirl and microwave for additional 20 seconds-1 minute

after microwaving, add 5uL ethidium bromide solution per 100mL

pour in gel – takes about 20 minutes to set

small gel: 50mL

medium gel: 75mL

large gel: 100mL

6X DNA sample buffer

3.75mL glycerol

6.25mL ddH2O

add a pinch of xylene cyanol and a pince of bromophenol blue

make sure to clean up extra spilled XC and BPB

can be stored at RT

Use 2uL dye per 10uL sample on an agarose gel