SDS-PAGE gels

Running gel % 8% 10% 12% 4% stack

Acrylamide 2.7mL 3.3mL 4mL 0.8mL

Buffer 2.5mL 2.5mL 2.5mL 1.5mL (stack buffer)

Water 4.8mL 4.2mL 3.5mL 3.7mL

10% APS 100uL 100uL 100uL 60uL

TEMED 10uL 10uL 10uL 6uL

10% APS

1g ammonium persulfate

to 10mL with ddH2O

store at 4 for 1-2 months.