For Cre

 

Primers:

Cre F   GCTAAACATGCTTCATCGTCGG

Cre R   GATCTCCGGTATTGAAACTCCAGC

 

Taq polymerase (Promega)

Buffer E (Epicentre technologies)

 

Program AEW in Anne WestÕs machine:

94û 2 minutes

 

(30 cycles for next 3 steps)

94û 30 seconds

55û 45 seconds

72û 30 seconds

 

72û 8 minutes

4û   hold

 

controls:

+ = Cre = 500 bp

- = wt = nothing