Protocols

2D Agarose gels Animal Ordering Baculo purification Baculovirus Ca Phos Transfection CaP-293T CaP CaP2 CaRF southerns Caspase9 purify CrePCR Cryo sections Ctxcx Dissection Dicer Production of     siRNAs EDTA and NaOH

ES cell genomic Flp transfection freezing cells Gel Shift genomicDNA genotyping PCR Glial coated CS GST Protocol Homemade ECL ICC LB  and plates MEF2 immunostaining MEF2 RNAi MEG Competent bacteria mouse calendar Mouse Care Neuronal depol and luc assays Northern pc12 pF Phenol

plating cortical neurons primer ordering Q RT-PCR QRT-PCR2 QRT-PCR2b QRT-PCR3 quikchange.pdf SDS-PAGE recipes SDS-PAGE gels shRNA lentiviral constructs sign up sheet SOBLabStockSolution-4.pdf Southern Blotting splitting 293T cells Splittingp Tae and agarose gel Transformation Viral prep2 western1samples western2gels western3blotting western recipes yeastprotein

Many of our studies address the mechanisms by which calcium signaling pathways regulate the function of transcription factors in neurons. Shown above, the calcium-selective transcription factor CaRF (red) is found in the nucleus (blue) of hippocampal neurons (stained green for the marker protein MAP2) but is not detected in glial cells.